cd3 microbeads (Miltenyi Biotec)

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Miltenyi Biotec cd3 microbeads
Cd3 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 549 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3/CD3+MicroBeads%2C+human/pm42876290-322-5-7
Average 96 stars, based on 549 article reviews

cd3 microbeads - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Tissue Factor-inhibited Beclin1-autophagy via BCL2 overexpression suppresses the differentiation of human monocytes into mature osteoclasts
Article Snippet: .. PBMCs were incubated with CD3 (130-050-101, Miltenyi), CD19 (130-050-301, Miltenyi) MicroBeads and NKp46 (CD335)-coupled anti-Biotin MicroBeads (130-125-207/130-090-485, Miltenyi) on LS column (130-042-401, Miltenyi) for MicroBead sorting, which aimed to deplete mode. .. Subsequently, after blocking non-specific binding using Fc-Receptor Blocking Solution (Human TruStain FcXTM, BioLegend, San Diego, CA, USA), cells were resuspended in Dulbecco’s Phosphate-Buffered Sallines (DPBS) along with 10% bovine serum albumin (BSA) for FACS, and stained for CD14-APC (301808, Biolegend), CD16-FITC (360716, Biolegend) for 20 minutes on ice.

Article Title: Modified NK-92 cells, and therapeutic and diagnostic uses thereof
Article Snippet: .. Flow Cytometric Analysis NK92 cells were incubated with FITC-, PE-, APC- or Pacific Blue-conjugated monoclonal antibodies (mAbs) specific for the indicated antigens TCR-Vß8 (Biolegend), TCR-Vß21.3 (Beckman Coulter), CD3, CD8, CTLA4, PD1 (all from BD Biosciences) CD96 (Santa Cruz Biotechnol.), NKG2A, NKG2C (both from Miltenyi Biotec), TIGIT, NKG2D, NKp30, NKp44 and TIM-3 (all from Biolegend) for 15 min at 22° C. and washed afterwards. ..

Staining:

Article Title: Safety and efficacy of allogeneic umbilical cord-derived mesenchymal stem cell infusion for frailty: a phase 2, single-centre, randomised, open-label controlled trial.
Article Snippet: At the indicated time points, one aliquot of PBMCs was analysed by flow cytometry, and CD3 + cells were subsequently isolated from the remaining PBMCs using CD3 microbeads (Miltenyi Biotec) for p16 INK4a mRNA and mitochondrial function analyses. .. To check the composition of immune cell populations in patients after UC-MSC infusion, PBMCs were stained with antibody panels including CD45, CD3, CD56, CD19, CD14, CD4, and CD8 (Miltenyi Biotec). .. Dead cells were excluded using 7-AAD (Miltenyi Biotec).

Article Title: Safety and efficacy of allogeneic umbilical cord-derived mesenchymal stem cell infusion for frailty: a phase 2, single-centre, randomised, open-label controlled trial
Article Snippet: At the indicated time points, one aliquot of PBMCs was analysed by flow cytometry, and CD3 + cells were subsequently isolated from the remaining PBMCs using CD3 microbeads (Miltenyi Biotec) for p16 INK4a mRNA and mitochondrial function analyses. .. To check the composition of immune cell populations in patients after UC-MSC infusion, PBMCs were stained with antibody panels including CD45, CD3, CD56, CD19, CD14, CD4, and CD8 (Miltenyi Biotec). .. Dead cells were excluded using 7-AAD (Miltenyi Biotec).

Article Title: Upregulation of CD1d and ULBP3 on B cells from healthy donors and chronic lymphocytic leukaemia patients does not prime them for killing by γδ T cells
Article Snippet: .. PBMC or γδ T cell lines were stained with a dead cell stain (DCS; Fixable Viability Dye eFluor 506; eBiosciences) or propidium iodide (Miltenyi Biotec), human Fc blocking agent (Miltenyi Biotec) and the following mAbs: CD3 (clone HIT3A), CD19 (HIB19), CD1d (51.1), CD107a (H4A3), IFN-γ (4S.B3), TNF-α (Mab11) CTLA-4 (BNI3), TIM-3 (F38-2E2) and Vδ2 (B6) were purchased from Biolegend, CD5 (UCHT2) and Vδ1 (REA173) were purchased from Miltenyi Biotech; ULBP3 (166510) and LAG-3 (polyclonal IgG) were purchased from R&D Systems; PD-1 (EH12.1) was purchased from BD Pharmingen; and Vδ3 (P11.5B) was purchased from Coulter Immunotech. .. Following staining, the cells were acquired on a BD FACSCanto II flow cytometer (BD Biosciences) and analysed using FlowJo v10 software.

Article Title: Upregulation of CD1d and ULBP3 on B cells from healthy donors and chronic lymphocytic leukaemia patients does not prime them for killing by γδ T cells.
Article Snippet: .. PBMC or γδ T cell lines were stained with a dead cell stain (DCS; Fixable Viability Dye eFluor 506; eBiosciences) or propidium iodide (Miltenyi Biotec), human Fc blocking agent (Miltenyi Biotec) and the following mAbs: CD3 (clone HIT3A), CD19 (HIB19), CD1d (51.1), CD107a (H4A3), IFN-γ (4S.B3), TNF-α (Mab11) CTLA-4 (BNI3), TIM-3 (F38-2E2) and Vδ2 (B6) were purchased from Biolegend, CD5 (UCHT2) and Vδ1 (REA173) were purchased from Miltenyi Biotech; ULBP3 (166510) and LAG-3 (polyclonal IgG) were purchased from R&D Systems; PD-1 (EH12.1) was purchased from BD Pharmingen; and Vδ3 (P11.5B) was purchased from Coulter Immunotech. .. Following staining, the cells were acquired on a BD FACSCanto II flow cytometer (BD Biosciences) and analysed using FlowJo v10 software.

Blocking Assay:

Bioprocessing:

Gradient Centrifugation:

Article Title: Method for producing t cells modified by chimeric antigen receptor
Article Snippet: .. Peripheral blood mononuclear cells (PBMCs) obtained by density gradient centrifugation were sorted by using CD3 (purchased from Miltenyi Biotechnology Co., Ltd., Germany, catalog No.: 130-050-101). ..

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